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发现一种以结构和序列为基础的蛋白质聚类为动力的热稳定塔加4-聚酶

JiaJun Chen^1,2, Dawei Ni^1,2, Yingying Zhu^1,2

¹State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China.

Journal of agricultural and food chemistry

|August 12, 2024

概括

一种新的酶,Thar-T4Ease,有效地将d-果糖转化为功能甜味剂d-塔加. 这种热稳定酶为经济高效的d-塔加生产提供了一个有前途的途径.

科学领域:

生物化学生物化学
酶学是一种酶学.
生物技术是生物技术.

背景情况:

d-Tagatose是一种功能性甜味剂,具有显著的生理益处.
酵素生产d-塔加为化学合成提供了一个可持续的替代方案.

研究的目的:

发现和描述一种能够将d-果糖转化为d-塔加的新型酶.
研究该新型酶的酶性质和工业应用潜力.

主要方法:

生物信息分析包括结构相似性搜索和蛋白质聚类以识别新型酶.
酶的重组表达和生化特征,包括确定最佳条件和动力学参数.
酶活性测定,热稳定性测试和转化率测量.
分子对接和位点定向突变发生,以确定关键的催化残留物.

主要成果:

一种新型的tagatose 4-epimerase (Thar-T4Ease) 已从一个 *Thermoprotei* 考古团中鉴定出来.
该酶在pH8.5和85°C的温度下表现出最佳活性,并具有Ni2+辅因子.
观察到高温稳定性 (t_1/2 = 198小时在80°C),d-果糖转化率为18.9%.
通过计算和实验方法确定了对催化活动至关重要的关键残留物.

结论:

Thar-T4Ease是一种高度稳定和高效的酶,用于从d-果糖中产生d-塔加.
这一发现扩大了已知的C4-表皮酶酶的范围.
这些发现为具有成本效益和可持续的d-塔加工业生产铺平了道路.

关键词:

这就是d-果糖.d-塔加托糖的使用.蛋白质聚类蛋白质的聚类.结构相似性搜索结构相似性搜索塔加-4-表皮酶的使用

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