Prdm1积极调节肝脏1组ILCs癌症免疫监测,并保持功能异质性
Jitian He1,2, Le Gao1, Peiying Wang1
1Institute of Medical Engineering & Translational Medicine, Tianjin University, Tianjin, China.
eLife
|August 12, 2024
概括
Prdm1对肝脏免疫细胞功能至关重要,影响癌症转移. 它在常规的自然杀手 (cNK) 细胞和1型先天性淋巴细胞 (ILC1s) 中缺乏,会损害抗瘤免疫力.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 在瘤学瘤学.
背景情况:
- 一组先天性淋巴细胞 (ILCs),包括常规的自然杀手 (cNK) 细胞和1型先天性淋巴细胞 (ILC1s),对肝脏免疫至关重要.
- 这些细胞通过细胞因子分泌和直接杀死细胞来调节肝脏的免疫微环境.
- 了解肝脏cNK细胞和ILC1s的转录因子调节是推动肝癌免疫疗法的关键.
研究的目的:
- 研究转录因子Prdm1在肝脏cNK细胞和ILC1s中的调节作用.
- 阐明Prdm1缺乏对肝癌进展和免疫细胞功能的影响.
- 为了确定肝癌免疫治疗的潜在治疗点.
主要方法:
- 使用条件淘汰赛小鼠模型 (Ncr1驱动) 来研究Prdm1的功能.
- 使用体内细胞毒性模型评估cNK细胞杀死功能.
- 分析了细胞因子分泌 (IFN-γ,granzyme B,perforin) 并进行了单细胞RNA测序 (scRNA-seq).
主要成果:
- Prdm1调节cNK细胞的组成和成熟.
- Prdm1 缺乏没有影响cNK细胞的杀死,但导致癌症转移的增加.
- 缺乏Prdm1的cNK细胞和ILC1s显示IFN-γ,大酶B和穿孔素的分泌量显著降低.
- scRNA-seq数据表明Prdm1维持cNK细胞和ILC1s的功能子集,并促进细胞与细胞的通信.
结论:
- Prdm1在肝脏cNK细胞和ILC1s.中发挥着一种新的调节作用.
- Prdm1 缺乏会影响肝脏的抗瘤免疫反应,促进转移.
- 向Prdm1为开发针对肝癌的新型免疫疗法提供了一个有前途的战略.
相关概念视频
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
T Cell Types and Functions
966
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
966
Regulation of Hematopoietic Stem Cells
3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
Liver Regeneration
3.2K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.2K


