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宿主驱动的选择,通过对Xanthomonas的比较分析揭示了III型分泌效应体,揭示了新的公认的效应体

Yao Xiao^1,2, Shatrupa Ray¹, Saul Burdman²

¹Department of Plant Pathology and Weed Research, Agricultural Research Organization-Volcani Institute, Rishon LeZion, Israel.

|August 12, 2024

概括

克桑托莫纳斯细菌使用特定的效应蛋白来感染植物. 这项研究表明,宿主植物塑造了这些细菌效应体,其中一些效应体被番茄识别,影响了病原体的毒性.

科学领域:

植物病理学植物病理学
微生物遗传学微生物遗传学
细菌病原体的产生

背景情况:

桑托莫纳斯物种是专门的植物病原体,采用III型分泌系统,向宿主细胞输送效应蛋白.
这些效应蛋白的组成,称为效应组,在物种之间有所不同,并被假设为驱动宿主专业化.

研究的目的:

为了分析与Solanaceae和Brassicaceae植物相关的Xanthomonas物种的效能体.
调查宿主衍生选择在塑造物种特异性效应组中的作用.
实验测试引入外来效应物对病原体毒性和宿主识别的影响.

主要方法:

对300多个Xanthomonas基因组进行比较基因组分析,以确定效应组.
在异构的Xanthomonas菌株 (X. campestris pv. 新型效应剂的引入. 坎普斯特里斯和X.欧维西卡托里亚PV. 欧维西卡托里亚).
对宿主植物 (白菜,西红,胡) 的病毒性测定和殖民研究.
通过Agrobacterium介导的短暂表达来确认效应体的识别.

主要成果:

确定了特定物种的效因子体特征,但对于Solanaceae或Brassicaceae宿主没有明显的特征.
观察到宿主偏差在效应器的存在/缺席.
引入特定的效应因子 (XopI,XopAC,XopJ5),在目标宿主中差异影响病毒毒性和殖民.
番茄识别了XopAC和XopJ5的效应因子,引起了过敏反应.

结论:

宿主衍生的选择在塑造Xanthomonas的物种特异性效应体中发挥着重要作用.
番茄识别了XopAC和XopJ5的效应因子,这有助于宿主-病原体相互作用.
对效能体的实验操纵为细菌适应和宿主专业化提供了见解.

关键词:

细菌病原体是一种细菌病原体.病原体的作用者是病原体的作用者.植物对病原体的识别

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