作为牛皮的有效分子认证标记,Cyt b基因是有效的牛皮分子认证标记
N Pavithra1, S Chris Felshia2, V John Sundar3
1Microbiology Division, CSIR - Central Leather Research Institute, Adyar, Chennai 20, Tamil Nadu, India; Department of Leather Technology, A.C. Tech, Anna University, Guindy, Chennai 20, Tamil Nadu, India.
Mitochondrion
|August 12, 2024
概括
真正的牛皮识别对商业至关重要. 这项研究开发了一种精确的RT-PCRTaqMan测定方法,使用线粒体DNA (mtDNA) 准确检测真牛皮,有助于皮革认证.
科学领域:
- 兽医科学 兽医科学 兽医科学
- 分子生物学分子生物学
- 法医科学 法医科学 法医科学
背景情况:
- 认证真正的牛皮对于皮革行业和全球贸易至关重要.
- 目前用于皮革认证的方法可能缺乏精度或效率.
- 对可靠的方法来识别真实的牛皮的需求正在增加.
研究的目的:
- 开发和验证一种高度特定和敏感的测试方法,用于验证牛皮的真实性.
- 在皮革产品中利用线粒体DNA (mtDNA) 进行精确的物种识别.
主要方法:
- 设计针对特定物种的原料和探针,以向线粒体DNA (mtDNA) 的细胞染色体b (Cyt b) 区域.
- 从各种牛皮样本 (Bos taurus和Bos indicus) 中提取mtDNA.
- 基于实时PCR (RT-PCR) 的TaqMan测定用于DNA分析.
主要成果:
- 开发的RT-PCRTaqMan试验证明了牛皮识别的高特异性.
- 该测试成功地放大并识别了成品皮革样本中的mtDNA.
- 测试的确定的检测极限被确定为0.1 ng的DNA.
结论:
- RT-PCR TaqMan 试验提供了一种可靠和开创性的方法来验证真牛皮的真实性.
- 来自成品皮革的可放大mtDNA是物种识别的可行目标.
- 这种测试通过确保产品真实性和经济完整性来支持皮革行业.
更多相关视频
15:57Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
Published on: July 11, 2015
12.2K
09:04Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
7.8K
相关概念视频
01:16Cell Lines
7.3K
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
7.3K
01:17Karyotyping
59.5K
Overview
59.5K
01:11Fibril-associated Collagen
2.5K
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
2.5K
01:20The Central Dogma
20.9K
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
20.9K