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Updated: Jun 17, 2025

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

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两个阿斯巴达酸盐之间共享的低屏障质子作为一个变形开关,改变β-乳酸酶BlaC的基质特异性

Jing Sun1, Aimee L Boyle1, Steffen Brünle2

  • 1Macromolecular Biochemistry, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, the Netherlands.

International journal of biological macromolecules
|August 12, 2024
PubMed
概括

在Mycobacterium tuberculosis BlaC中的谷氨酸残留物对于水解ceftazidime并非必不可少. 高 pH 诱导 BlaC 中的构造转换,增强 ceftazidime 的水解并改变基质的特异性.

关键词:
形状的变化是形状的改变.酶动力学 酶动力学对于pH值的敏感度

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