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优化批量种植和扩大Bacillus thuringiensis的规模,以生产高产的聚3-基酸盐-co-3-基酸盐)
Lovely1, Sanjay Kumar1, Ashok Kumar Srivastava1
1Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
Bioresource technology
|August 12, 2024
概括
优化了B. thuringiensis的批量种植,使用酸增强的聚3-基酸-co-3-基酸 (PHBV) 生产. 扩展到70L生物反应器证明了可持续应用的高效生物聚合物产量.
科学领域:
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
- 微生物发酵 微生物发酵
背景情况:
- 聚酸酸盐 (PHAs) 是一种可生物降解的聚合物,具有多种应用.
- 优化微生物发酵是具有成本效益的PHA生产的关键.
- 硫杆菌 (B. thuringiensis) 是PHA合成的潜在宿主.
研究的目的:
- 优化B. thuringiensis的批量种植,以提高聚3-基酸-co-3-基酸 (PHBV) 的生产.
- 扩大优化发酵过程的规模,使其具有工业相关性.
- 为了对产生的PHBV进行潜在应用的表征.
主要方法:
- 使用葡萄糖和甘油混合基质进行B. thuringiensis的批量栽培.
- 在最佳的度和时间点添加酸作为电子受体.
- 使用功率/体积 (P/V) 标准,从7L升级到70L的生物反应器.
- 使用核磁共振 (NMR),里埃变形红外光谱 (FTIR),差分扫描热度计 (DSC) 和热重力计分析 (TGA) 进行PHBV的表征.
主要成果:
- 在特定的种植时间添加1.2g/L酸,产生了8g/L生物质和3.57g/LPHBV与0.805g/L3-基酸.
- 成功升级到70L生物反应器,达到PHBV和生物质度分别为3.57g/L和7.15g/L的最大值.
- 综合性表征阐明了产生的PHBV的结构,热性能和稳定性.
结论:
- 统计优化的批量种植和扩大规模显著提高了B. thuringiensis.的PHBV产量.
- 标志性的PHBV表现出适用于各种社会应用的特性.
- 这项研究强调了优化发酵对可持续生物聚合物生产的潜力.
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