带有集成传感器的微生理系统,用于研究脉冲电场的影响
Neringa Bakute1, Eivydas Andriukonis2, Kamile Kasperaviciute2
1Laboratory of Bioelectrics, State Research Institute, Center for Physical Sciences and Technology, Sauletekio Ave. 3, 10257, Vilnius, Lithuania. neringa.bakute@ftmc.lt.
Scientific reports
|August 12, 2024
概括
这项研究引入了用于脉冲电场 (PEF) 细胞研究的非静态测量硫 (OSTE) 微芯片,提供集成监测. 微芯片有效地用PEF对哺乳动物细胞进行治疗,同时可以在现场进行生理测量.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 材料科学 材料科学 材料科学
背景情况:
- 在先进的细胞研究中,聚甲基 (PDMS) 微芯片缺乏化学和机械抗性.
- 将分析测量设置集成到微生理系统 (MPS) 中具有重大挑战.
研究的目的:
- 为使用脉冲电场 (PEF) 进行受控细胞研究开发和验证基于非静电测量乙烯 (OSTE) 的微芯片.
- 集成用于PEF和体电阻 (TEER) 测量的电极,以及在现场pH和氧气监测.
主要方法:
- 制造基于OSTE的微芯片,集成电极和传感器.
- 在停止流和连续流模式下对C6电池应用PEF.
- 使用光染料 (DapI) 评估细胞活力和透性.
- 在PEF处理过程中在现场监测氧度和pH值变化.
主要成果:
- 在1.8kV/cm以16个脉冲 (停止流) 和64个脉冲/细胞 (连续流) 实现DapI的最大细胞透率,而不会影响活力.
- 集成的传感器检测到氧度变化和转向性在PEF处理后的pH值.
结论:
- OSTE微芯片作为一种可行的微生理系统 (MPS),用于对哺乳动物细胞进行PEF处理.
- 集成的传感能力允许在PEF应用过程中实时现场生理监测.
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