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Updated: Jun 17, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

循环RNA NFIX通过调节miR-214-3p/TRIAP1轴,在非小细胞肺癌中作为瘤基因起作用

Guohua Liu¹, Hanbing Shi¹, Hongyan Zheng¹

¹Department of Respiratory and Critical Care Medicine, The Third Affiliated Hospital of Qiqihar Medical College, Qiqihar, China.

The clinical respiratory journal

|August 12, 2024

概括

循环RNA NFIX (circRNA NFIX) 通过对miRNA-214-3p进行下调和对TRIAP1.1.进行上调来促进非小细胞肺癌 (NSCLC). 沉默circRNA NFIX抑制NSCLC细胞增殖,并诱导细胞亡.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因规则基因规则

背景情况:

循环RNA NFIX (circRNA NFIX) 被认为是质瘤中的瘤基因.
在非小细胞肺癌 (NSCLC) 中,circRNA NFIX的表达和功能在很大程度上仍未被描述.

研究的目的:

研究NSCLC中circRNA NFIX的表达特征.
阐明circRNA NFIX在NSCLC细胞增殖和亡中的功能作用.
探索涉及NSCLC中circRNA NFIX,miRNA-214-3p和TRIAP1的分子机制.

主要方法:

定量逆转录-聚合酶链反应 (qRT-PCR) 用于评估circRNA NFIX,miRNA-214-3p和TRIAP1.1的表达水平.
生物信息学预测 (星基) 和双露西法酶记者测试验证了circRNA NFIX/TRIAP1和miRNA-214-3p.之间的相互作用.
分别进行MTT测定和流细胞计,以评估细胞增殖和细胞亡.
西方斑分析用于测量与亡相关的蛋白质表达.

主要成果:

发现circRNA NFIX在NSCLC组织和细胞系中受到上调,而miRNA-214-3p在NSCLC组织和细胞系中受到下调.
抑制circRNA NFIX抑制NSCLC细胞增殖和诱导细胞亡,与增加的miRNA-214-3p表达相关.
确定TRIAP1是miRNA-214-3p的标,其表达受到miRNA-214-3p的负调节. 过度表达TRIAP1逆转了miRNA-214-3p模仿对NSCLC细胞的影响.

结论:

通过circRNA NFIX/miRNA-214-3p/TRIAP1轴,抑制circRNA NFIX沉默抑制NSCLC细胞增殖并诱导细胞亡.
这个轴代表了NSCLC的潜在诊断和治疗目标.

关键词:

在NSCLCLC中,我们可以看到.这里是 TRIAP1 .循环RNA NFIXIX 的时间.微RNA-214-3p 的情况.

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