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减弱的总反射里埃变换红外光谱学揭示了克隆日本结状植物中的环境特异性表型

Claire A Holden¹, Martin McAinsh², Jane E Taylor²

¹Lancaster Environment Centre, Lancaster University, Lancaster, LA1 4YQ, UK. c.holden6@lancaster.ac.uk.

BMC plant biology

|August 12, 2024

概括

根据环境条件,日本结呈现出明显的光谱差异,使用减弱总反射里埃变换红外光谱学 (ATR-FTIR) 来确定. 这些与植物压力和环境因素 (如光质) 相关的光谱变化,可能解释了它的入侵成功.

科学领域:

植物生物学植物生物学
频谱学是一种光谱学.
生态生态学生态生态学

背景情况:

日本 (Reynoutria japonica) 是一个具有广泛分布的入侵物种.
之前的研究表明,ATR-FTIR光谱学可以区分区域植物变异.
对日本结状的光谱差异的环境贡献仍然不清楚.

研究的目的:

调查各种环境息地对日本结状植物生长和光谱形状的影响.
确定关键的分子生物标志物和与环境压力相关的光谱区域.
使用ATR-FTIR光谱学开发植物生理参数的预测模型.

主要方法:

通过操纵光比 (R:FR),水,和微量营养素,创造了八个不同的生长环境.
使用化学测量 (PCA,LDA,SVM,PLSR) 分析了植物生长,光合作用参数和ATR-FTIR光谱.
确定了关键波数和分子生物标志物,并开发了根水潜力 (RWP) 的预测模型.

主要成果:

与SVM相结合的ATR-FTIR光谱学成功地从不同环境中的植物中分辨出光谱.
PCA负载确定了细胞壁碳水化合物作为跨物种植物压力的常见生物标志物.
一个PLSR模型以高精度 (R2 = 0.8) 预测了根水潜力,证明了ATR-FTIR作为传感器的潜力.

结论:

日本结表现出环境诱导的表型,具有可测量的ATR-FTIR光谱差异.
由生物分子变化表明的高环境可塑性,可能有助于其侵入性成功.
光质 (R:FR) 对于光谱反应至关重要;保存的生物标志物表明可以监测物种间的植物健康和压力.

关键词:

里埃变换红外光谱学红外光谱学引入的物种是引入的物种.日本的结状植物.现型性可塑性现型性可塑性主要组件分析的主要组件分析.支持向量机器.

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