在人类肌肉膜光滑肌肉细胞系中,CD38/cADPR介导的信号传递,PHM1
Soner Dogan1,2, Timothy F Walseth3, Bilge Guvenc Tuna4
1Department of Medical Biology, School of Medicine, Yeditepe University, Istanbul, Turkey.
IUBMB life
|August 13, 2024
概括
由CD38蛋白介导的循环ADP-ribose (cADPR) 信号传递,在孕妇人类肌肉细胞中调节细胞内的作用. 这一途径参与由激动剂刺激的子宫收缩.
科学领域:
- 身体生理学 身体生理学
- 细胞生物学 细胞生物学
- 生物化学 生化学
背景情况:
- 循环ADP-ribose (cADPR) 在光滑肌细胞中充当调节的第二信使.
- CD38蛋白具有ADP-ribosyl环酶和cADPR酶的活性,这对cADPR代谢至关重要.
- 以前的研究表明,雌激素和孕激素调节肌肉体中的CD38表达.
研究的目的:
- 为了研究CD38/cADPR信号传递在怀孕人类肌肉细胞的收缩激素刺激期间细胞内调节中的作用.
- 为了利用永生孕妇人体肌肉细胞 (PHM1) 作为研究人体肌肉细胞的模型.
主要方法:
- 西部斑点和免疫光检测证实了PHM1细胞中CD38的表达.
- 生物化学试验量化了ADP-ribosyl环酶和cADPR酶活动.
- 测量细胞内 ([Ca2+]i) 对催产素,PGF2α和ET-1的反应,有或没有cADPR对抗剂8-Br-cADPR.
主要成果:
- 在PHM1细胞膜上证实了CD38表达和酶活性.
- 收缩性激动剂 (催产素,PGF2α,ET-1) 诱导了[Ca2+]i反应.
- 8-Br-cADPR显著减弱了激素诱导的[Ca2+]i反应的20-46%.
结论:
- 子宫收缩激动剂通过CD38 / cADPR信号传递部分调解其作用,以提高细胞内.
- 建议这种途径有助于子宫收缩.
- 由于有限的原始组织可用性,PHM1细胞为研究人类肌子膜提供了有价值的体外模型.
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