相关实验视频
Updated: Jun 17, 2025

07:12
A Colorimetric Method for Measuring Iron Content in Plants
Published on: September 7, 2018
22.0K
延长型阴5 (HY5) 和POPEYE (PYE) 调节植物细胞间的铁运输
Samriti Mankotia1, Abhishek Dubey1,2, Pooja Jakhar1
1Department of Biological Sciences, Indian Institute of Science Education and Research (IISER), Mohali, Punjab, India.
Plant, cell & environment
|August 13, 2024
概括
一种转录因子HY5 (HY5) 与POPEYE (PYE) 相互作用,调节植物中的铁平衡. 它们一起控制铁的运输和储存,确保在缺铁情况下的植物健康.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 植物需要严格调节铁 (Fe) 恒温来维持生存.
- 虽然了解了Fe的吸收,但Fe运输和储存的上游监管者仍然不清楚.
- 众所周知,POPEYE (PYE) 是一种转录因子,对于Arabidopsis的Fe平衡至关重要.
研究的目的:
- 为了研究延长型HYPOCOTYL5 (HY5) 在植物铁恒温中的作用.
- 阐明HY5和PYE在调节铁的运输和储存方面的相互作用.
- 为了确定Fe运输和储存的上游转录调节器.
主要方法:
- 采用了分子,遗传和生物化学方法.
- 与Fe运输相关的基因表达的分析.
- 促进体结合测定和蛋白相互作用研究.
主要成果:
- HY5与PYE相互作用,它们在Fe缺乏反应中具有共同的作用.
- 无论是HY5还是PYE都抑制了Fe运输基因 (例如,YSL3,FRD3,NPF5.9,YSL2,NAS4,OPT3).
- HY5直接与这些基因的促进体结合,调节细胞间Fe运输.
结论:
- HY5在植物铁平衡中起着重要作用.
- HY5与PYE相互作用,并独立地调节Fe运输.
- 这项研究揭示了Fe在植物中的运输和储存的新兴上游监管机制.
相关概念视频
Short-distance Transport of Resources
15.9K
Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
15.9K
Key Elements for Plant Nutrition
18.7K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
18.7K
Protein Transport to the Inner Chloroplast Membrane
2.1K
Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
2.1K
Regulation of Transpiration by Stomata
28.1K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
28.1K
Water and Mineral Acquisition
32.8K
Specialized tissues in plant roots have evolved to capture water, minerals, and some ions from the soil. Roots exhibit a variety of branching patterns that facilitate this process. The outermost root cells have specialized structures called root hairs that increase the root surface, thus increasing soil contact. Water can passively cross into roots, as the concentration of water in the soil is higher than that of the root tissue. Minerals, in contrast, are actively transported into root cells.
32.8K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K

