破坏单纯疹病毒2型pUL21酸化损害了细胞质核囊的二次包裹
Renée L Finnen1, Jamil H Muradov1, Valerie Le Sage1
1Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada.
Journal of virology
|August 13, 2024
概括
简单疹病毒2型 (HSV-2) 体蛋白pUL21的酸化对于病毒复制和二次包裹至关重要. 这一过程需要病毒激酶pUL13并与HSV-1不同,影响病毒传播.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 翻译后修改 翻译后修改
背景情况:
- 体蛋白pUL21在单纯疹病毒 (HSV) 感染中起到多功能作用.
- 翻译后的修改,如酸化,对于调节蛋白质功能和病毒生命周期至关重要.
- 之前已经观察到HSV-1和HSV-2之间的pUL21功能和修饰的差异.
研究的目的:
- 为了确定HSV-2pUL21.21上的酸化部位.
- 确定pUL21酸化在HSV-2复制和病毒成熟中的作用.
- 为了研究负责pUL21酸化的病毒激酶.
主要方法:
- 位点定向的突变发生,以识别血清残留物 (Ser251和Ser253) 作为HSV-2pUL21中的酸化位点.
- 对缺乏pUL21酸化的HSV-2突变病毒的生成和表型分析.
- 对病毒复制,细胞间传播和二次包裹缺陷的分析.
- 研究病毒氨酸/氨酸激酶pUL13在pUL21酸化中的作用.
主要成果:
- 在HSV-2pUL21链接区域的两个特定的血清残留物 (Ser251和Ser253) 被确定为酸化部位.
- 缺乏pUL21酸化的HSV-2病毒显示细胞细胞扩散和病毒复制减少.
- 在缺乏pUL21酸化的病毒中观察到细胞质核体的二次包裹缺陷.
- 在感染 HSV-2 突变病毒缺乏病毒激酶 pUL13 的细胞中,pUL21 酸化显著降低.
- 在感染HSV-1的细胞中没有检测到pUL21的酸化.
结论:
- 病毒酶pUL13对HSV-2pUL21的酸化对于有效的病毒复制和二次包裹至关重要.
- 在HSV-2 pUL21上发现的酸化部位在HSV-1 pUL21中不存在,这解释了pUL21功能的特定物种差异.
- 酸化pUL21对于细胞质核酸的二次包裹至关重要,这是疹病毒成熟的关键步骤.
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