由Si-Ni-San通过HIF-1路径调制识别的四种化合物尾酒的抗抑郁性质
Na An1, Dongxing Zhang2, Jile Xin1
1College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China.
Current computer-aided drug design
|August 13, 2024
概括
通过调节HIF-1信号通路,Si-Ni-San (SNS) 显示出抗抑郁的潜力. SNS中的关键化合物与AKT1和MAPK1相互作用,对氧化应激产生保护作用.
科学领域:
- 药理学 药理学是指药理学的学科.
- 传统中国医药 传统中国医药
- 神经科学是一个神经科学.
背景情况:
- 锡尼桑 (Si-Ni-San,简称SNS) 是一种具有已知的抗抑郁功能的传统中医 (TCM) 配方.
- 需要阐明SNS抗抑郁作用背后的精确分子机制.
研究的目的:
- 研究SNS在治疗抑郁症中的药理机制.
- 确定SNS的关键活性化合物和分子标.
- 探索新的方法来研究TCM.
主要方法:
- 网络药理学被用来确定SNS的关键途径和目标.
- 使用SwissTargetPrediction和分子对接来分析化合物-目标相互作用.
- 在体外测试 (CCK-8,霍克斯特染色,H2DCFDA,DPPH,细胞内西部) 评估了SNS化合物的保护作用,以防止皮质激素诱导的氧化应激在PC12细胞中.
主要成果:
- 网络药理学发现HIF-1信号通路对于SNS的抗抑郁作用至关重要.
- 在HIF-1通路内确定了包括AKT1和MAPK1在内的关键标蛋白.
- 来自SNS的奎尔塞丁,纳灵宁,利科哈尔科恩A和凯姆菲罗尔的尾酒显示了保护作用,减少了氧化应激,并在体外调节了关键蛋白质表达.
- 分子对接表明SNS尾酒与AKT1和MAPK1.1直接结合.
结论:
- 含有奎尔塞丁,纳灵宁,利科哈尔科恩A和凯姆菲罗尔的SNS尾酒具有抗抑郁的潜力.
- 通过与AKT1和MAPK1的直接相互作用来调节HIF-1信号通路是拟议的作用机制.
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