保存的cis元素使得在结节过程中通过NODULE INCEPTION通过NODULES WITH ACTIVATED DEFENSE1调节结节
Haixiang Yu1,2, Aifang Xiao1,3, Zhongmin Zou1
1National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
The Plant cell
|August 13, 2024
概括
在固定类植物 (NFC) 中,共生性固定涉及NAD1基因,由结开始 (NIN) 调节. 这种NIN-NAD1模块对于在植物结节中容纳根茎菌至关重要.
科学领域:
- 植物分子生物学 植物分子生物学
- 进化生物学是进化的生物学.
- 微生物学 微生物学
背景情况:
- 在固定类植物 (NFC) 中,共生性固是一种关键的农业特征.
- 结节的进化起源被假设涉及基因增加和损失,但分子证据很少.
- 有激活防御的结节1 (NAD1) 是结节的候选基因.
研究的目的:
- 研究NFC植物中NAD1的进化保存和功能.
- 确定控制NAD1在结节中的表达的调节元素.
- 阐明结节开始 (NIN) 蛋白在调节NAD1.1中的作用.
主要方法:
- 植物基因组的生物信息分析以确认NAD1的存在并确定cis调节元素.
- 定量逆转录PCR分析NAD1在结节中的基因表达.
- 促进体删除分析和位点定向的突变发生,以确定功能结节相关的 cis 调节元件 (NACE).
- 电泳运动转移测定 (EMSA) 证实NIN与NACEs的结合.
- 在Lotus japonicus中分析CRISPR-Cas9诱导的LjNAD1突变.
主要成果:
- 证实NAD1是一种NFC特异性基因,仅以结节形式表达.
- 在LjNAD1促进体中发现了三种保守的结节相关的cis-regulatory元素 (NACE1-3).
- NACE1和NACE2分别显示了Fabales和Papilionoideae的特异性,而NACE3则在NFC植物中得到保留.
- 结节开始 (NIN) 直接与NACE1-3结合,激活NAD1的表达.
- LjNAD1突变体表现出异常的共生体形成和细菌体分解,类似于Mtnad1和Mtnin突变体.
结论:
- NIN-NAD1调控模块对于NFC植物结核中的树根生物适应至关重要.
- 通过NIN调节的NAD1代表了结节的关键进化适应.
- 这项研究提供了分子证据,支持NFC植物中结节的进化模型.
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