由代谢振荡器驱动的脂质滴含量中的节奏在整个进化过程中都保持不变
Paula M Wagner1,2, Mauricio A Salgado1,2, Ornella Turani3
1CIQUIBIC-CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, 5000, Córdoba, Argentina.
Cellular and molecular life sciences : CMLS
|August 13, 2024
概括
脂质滴滴 (LD) 含量的代谢节奏在整个进化过程中都保持不变. 这项研究发现,细菌,虫和人体细胞中LD的每日振荡受到细胞能量需求和氧化还原振荡器的调节.
科学领域:
- 细胞生物学 细胞生物学
- 时间生物学 时间生物学
- 代谢调节 代谢调节 代谢调节
背景情况:
- 细胞生物钟控制着每天的生理和行为节奏.
- 分子时钟和氧化还原振荡器可以协调细胞节律.
- 反氧振荡对于适应氧气变化至关重要,它调节了每天的糖脂代谢.
研究的目的:
- 为了研究脂肪滴 (LD) 含量中代谢节奏的进化存在.
- 为了探索 LD 振荡在 prokaryotic 和 eukaryotic 生物体中的保存.
主要方法:
- 在Pseudomonas aeruginosa (细菌) 和Caenorhabditis elegans (真核生物) 中研究了温度周期同步的LD含量的时间振荡.
- 在德克萨脉冲后分析了血清冲击同步的人类胚胎脏 (HEK 293) 和脑瘤细胞 (T98G,GL26).
- 研究了糖原合成酶激酶-3 (GSK-3) 抑制和 LIPIN-1 敲击对 T98G 细胞中 LD 振荡的影响.
主要成果:
- 在细菌,线虫和人类细胞系中观察到LD含量的持续时间振荡.
- LD振荡与细胞能量恒温有关,并受到氧化还原振荡器的影响.
- 在同步的T98G细胞中,GSK-3抑制和LIPIN-1敲击改变了LD振荡.
结论:
- 在各种进化尺度上,LD含量的代谢振荡高度保留.
- 这些节奏表明复杂性,调节和细胞组织的变化.
- LD内容振荡是蜂计时和能源管理的一个基本方面.
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