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在牙周炎中识别和验证与衰老和内质网膜压力相关的基因,使用竞争的内源性RNA网络

Xinran Feng¹, Da Peng², Yunjing Qiu³

¹State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Preventive Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, 710032, China.

|August 13, 2024

概括

这项研究确定了与衰老相关的基因和与牙周炎病原发生有关的内质网膜压力相关的基因. 它揭示了一个新的ceRNA网络,促进了对这种慢性炎症疾病的理解.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

牙周炎是一种影响牙周的慢性炎症性疾病.
人们越来越认识到衰老和内质网膜 (ER) 压力是牙周炎的关键因素.
了解涉及衰老和ER压力的分子机制对于牙周炎研究至关重要.

研究的目的:

为了确定与衰老相关的基因 (ARG) 和与牙周炎相关的内质网膜压力相关的基因 (ERG).
构建一个具有竞争力的内源性RNA (ceRNA) 网络,涉及与牙周炎中衰老和ER压力相关的mRNA,miRNA和lncRNA.
通过实验验证生物信息学发现.

主要方法:

从GEO,HAGR和GeneCards数据库中进行数据挖掘,以识别差异表达基因 (DEG),ARG和ERG.
使用MultiMiR和STARBase数据库构建一个mRNA-miRNA-lncRNA ceRNA网络.
权重基因共同表达网络分析 (WGCNA) 和单个样本基因组丰富分析 (ssGSEA) 用于模块探索和免疫透.
使用TEM,光显微镜,qRT-PCR和Western Blot在感染Porphyromonas gingivalis的PDLSCs中的实验验证.

主要成果:

确定了10个枢纽基因和5个DEmRNAs-ARGs-ERGs.
在牙周炎中建立与衰老和ER压力相关的ceRNA网络.
实验验证在很大程度上证实了生物信息学预测.

结论:

该研究成功地在牙周炎中建立了一个与衰老和ER压力相关的新型ceRNA网络.
这个网络为牙周炎的病原体提供了新的见解.
这些发现有助于更深入地了解牙周炎的分子基础.

关键词:

细胞内膜网膜应激压力是什么衰老的衰老衰老的衰老竞争的内源性RNA网络.牙周带干细胞是牙周带干细胞.牙周病是指牙周病的发生.

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