染色体重塑驱动的自活化诱导了口腔状细胞癌中的思普拉丁耐药性
Su Young Oh1, Jinkyung Kim1, Kah Young Lee1
1Department of Microbiology and Immunology, School of Dentistry, Kyungpook National University, Daegu, South Korea.
Cell death & disease
|August 13, 2024
概括
基因表达影响口腔癌的化学抵抗. 特定的基因组基因 (HIST3H2A/B) 促进自和抗西斯普拉丁抗性,而其他基因 (HIST1H3D) 则相反相关,提供潜在的预测标记.
科学领域:
- 分子瘤学分子瘤学
- 癌症遗传学 癌症遗传学
- 细胞生物学 细胞生物学
背景情况:
- 预测西斯在口腔状细胞癌 (OSCC) 的疗效是具有挑战性的,特别是在自方面.
- 染色体重塑基因在化学抵抗和自中所起的作用尚未完全理解.
研究的目的:
- 为了研究染色体重塑基因对OSCC. cisplatin耐药性的影响.
- 探索这些基因与自的相互作用.
- 在OSCC中识别化学抵抗的潜在生物标志物.
主要方法:
- 在对西斯普拉丁敏感 (UMSCC1) 和抗性 (UM-Cis) OSCC细胞中的基因表达的分析.
- 对选定基因组基因 (HIST1H3D,HIST3H2A,HIST3H2B) 的功能的增加和丧失研究.
- 评估自标志物和MiTF表达的评估.
- 患者组织中蛋白质表达的比较.
主要成果:
- 基因表达的显著差异,特别是参与核细胞组合的基因表达.
- HIST3H2A和HIST3H2B通过上调自而与西斯普拉丁耐药性正相关.
- HIST1H3D显示与西斯抗药的逆相关性.
- MiTF对HIST1H3D转录进行了负调节,表明了复杂的相互作用.
- 实验室检测结果在患者的OSCC组织中得到了验证.
结论:
- 通过HIST3H2A/HIST3H2B进行染色体重塑,在OSCC中促进了自和西斯普拉丁抗性.
- 一组染色体重塑基因显示出在OSCC活检中具有化学抵抗预测标记的潜力.
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