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Updated: Jun 17, 2025

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
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Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

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无标签追踪用于量化通过生物介质的纳米粒子扩散

Genevieve Schleyer1, Eann A Patterson2, Judith M Curran3

  • 1Department of Materials, Design & Manufacturing Engineering, University of Liverpool, Brownlow Hill, Liverpool, UK. G.Schleyer@liverpool.ac.uk.

Scientific reports
|August 13, 2024
PubMed
概括

这项研究使用了无标签的光学跟踪技术来测量金纳米粒子在生物介质中的扩散. 发现蛋白质度,电荷和直径会影响纳米粒子运动,从而推进纳米技术应用.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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