通过通过miR-615-5p调节ETS1表达,Circ-PITX1促进非小细胞肺癌的进展
Yang Guo1, Jianfang Pan1, Xiaofei Gao1
1Department of Pulmonary and Critical Care Medicine, Zhejiang Jinhua Guangfu Tumor Hospital, Jinhua, China.
Thoracic cancer
|August 14, 2024
概括
循环RNA垂体同源盒1 (circ-PITX1) 通过调节miR-615-5p/ETS1通路,促进非小细胞肺癌 (NSCLC) 的进展. 低circ-PITX1表达抑制了NSCLC中的瘤生长和转移.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 循环RNAs (circRNAs) 在人类癌症中至关重要.
- 在非小细胞肺癌 (NSCLC) 中,circRNA pituitary homeobox 1 (circ-PITX1) 的作用需要进行研究.
研究的目的:
- 研究NSCLC中circ-PITX1的生物功能和潜在机制.
主要方法:
- 定量逆转录-聚合酶连锁反应 (qRT-PCR) 用于RNA表达.
- 细胞测试 (CCK-8,殖民地形成,EDU,流细胞计,伤口愈合,Transwell) 来评估生物行为.
- 使用谷氨胺代谢,蛋白质水平,双化酶,RNA免疫沉 (RIP) 和异种移植模型来阐明机制.
主要成果:
- 在NSCLC组织和细胞中,Circ-PITX1过度表达.
- 对circ-PITX1的下调抑制了NSCLC细胞的增殖,运动性,谷氨酸代谢和瘤形成,同时促进了亡.
- 通过海绵化miR-615-5p,Circ-PITX1对ETS1进行了积极调节.
结论:
- 环-PITX1促进NSCLC的进展.
- 环-PITX1/miR-615-5p/ETS1轴是NSCLC病变发生的一个关键途径.
- Circ-PITX1可能成为NSCLC的潜在治疗点.
相关概念视频
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
Mitogens and the Cell Cycle
6.4K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.4K
Induced Pluripotent Stem Cells
4.0K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.0K
lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
The Retinoblastoma Gene
4.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K


