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通过一种高度选择性和敏感的光法质疑自然界中RNA酸的存在

Luo He¹, Yu Xiang¹

¹Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Tsinghua University, Beijing 100084, China.

Analytical chemistry

|August 14, 2024

概括

天然RNA酸 (PT) 是一个有争议的问题. 一种新的光方法在测试的细菌或人体细胞中没有发现显著的RNA PT,这挑战了以前广泛传播的自然RNA PT的说法.

科学领域:

分子生物学分子生物学
生物化学生化学
分析化学分析化学

背景情况:

DNA酸 (PT) 在 prokaryotes 中自然存在,但RNA PT 的自然存在仍然存在争议.
之前使用LC-MS/MS的研究报告了高水平的自然RNAPT,但由于其他RNA修饰的潜在干扰,这些发现受到争议.
需要一种独立的,可靠的方法来准确量化生物样本中的RNA PT.

研究的目的:

开发和验证一种高度选择性和敏感的基于光的方法来定量RNAPT.
为了研究从原生细胞和真核细胞的RNA样本中天然RNAPT的存在和丰度.
重新评估先前LC-MS/MS分析表明的自然RNAPT的广泛发生情况.

主要方法:

开发一种用于选择性检测的新型RNA PT特异性转化反应.
使用敏感光试验对RNA PT进行量化,检测极限为2.8 PPM-nt.
从细菌和人类细胞系使用开发的光方法分析总RNA.

主要成果:

开发的光测试显示出高选择性,区分RNA PT与RNA硫酶和蛋白质囊蛋白.
在检测到的细菌和人类RNA样本中没有观察到可检测的RNA PT信号,超过2.8 PPM-nt检测极限.
这些发现表明,在以前报告的水平 (>100 PPM-nt) 上,自然RNA PT不太可能广泛传播.

结论:

开发的光方法为RNA PT检测和量化提供了一个简单,低成本和可靠的工具.
这项研究使人们对测试生物体中RNA PT自然存在的广泛存在产生怀疑.
建议使用这种方法进行进一步的广泛查,以确定不同物种中RNA PT的自然发生.

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