各种样品储存条件和样品细菌污染对常规生物化学参数度的影响
Amara Gojković1, Sandra Vladimirov1, Tamara Antonić1
1University of Belgrade, Faculty of Pharmacy, Department of Medical Biochemistry, Belgrade.
Journal of medical biochemistry
|August 14, 2024
概括
这项研究研究了储存条件和冷解周期如何影响EDTA和NaF血中的生物化学参数. 还评估了细菌污染,突出了分析前阶段的漏洞.
科学领域:
- 临床化学 临床化学
- 实验室医学 实验室医学
- 分析生物化学 分析生物化学
背景情况:
- 在实验室测试中,预分析阶段至关重要,包括样本采集,运输和储存.
- 储存条件和冷解周期的变化可能会影响生物化学测试结果的准确性.
- 样本处理过程中的细菌污染是实验室诊断中的潜在混因素.
研究的目的:
- 评估不同储存条件下的血中关键生物化学参数的稳定性.
- 为了确定重复的冷解周期对分析剂稳定性的影响.
- 在常规实验室样本中调查细菌污染的流行情况.
主要方法:
- 血样本 (EDTA和NaF) 在+4°C和-20°C保存.
- 分析物度 (胆固醇,葡萄糖,甘油三,尿素,白蛋白) 用标准测定方法进行测量.
- 结解周期 (FTC) 每天应用五天.
- 使用16S rRNA测序确定了细菌污染.
主要成果:
- 分析物的稳定性因储存温度和冷-解周期的数量而异.
- 经过多次FTC,特定的生物化学参数显示降解.
- 在一些样品中检测到细菌污染,这表明样品处理可能存在问题.
结论:
- 储存条件和冷解周期显著影响生物化学标记物的稳定性.
- 要确保可靠的实验室结果,必须仔细管理分析前阶段.
- 对细菌污染的监测对于准确的诊断测试至关重要.
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