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IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

1.2K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
1.2K
Photoelectric Effect02:26

Photoelectric Effect

29.5K
When light of a particular wavelength strikes a metal surface, electrons are emitted. This is called the photoelectric effect. The minimum frequency of light that can cause such emission of electrons is called the threshold frequency, which is specific to the metal. Light with a frequency lower than the threshold frequency, even if it is of high intensity, cannot initiate the emission of electrons. However, when the frequency is higher than the threshold value, the number of electrons ejected...
29.5K
X-ray Imaging01:24

X-ray Imaging

5.4K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
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Updated: Jun 17, 2025

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

9.7K

在双光子电压成像上的光学约束.

F Phil Brooks1, Hunter C Davis1, J David Wong-Campos1

  • 1Harvard University, Department of Chemistry and Chemical Biology, Cambridge, Massachusetts, United States.

Neurophotonics
|August 14, 2024
PubMed
概括

在体内,双光子 (2P) 电压成像需要比单光子 (1P) 成像更多的照明功率,同时记录只限于少数神经元. 对大脑深处许多神经元的高SNR 2P成像,需要在遗传编码电压指示器 (GEVI) 或新型成像技术方面取得进展.

科学领域:

  • 神经科学是一个神经科学.
  • 生物物理学的生物物理.
  • 光学成像技术的成像

背景情况:

  • 在体内神经电路研究中,基因编码的电压指示器 (GEVIs) 是至关重要的.
  • 一光子 (1P) 和两光子 (2P) 电压成像的比较性能仍然不太清楚.

研究的目的:

  • 描述1P和2P电压成像的光学和生物物理限制.
  • 为了比较常见的GEVI在1P和2P激发下的成像特性.

主要方法:

  • 在1P和2P照明下测量了GEVI的亮度和电压灵敏度.
  • 评估光衰变与深度在小鼠大脑组织.
  • 开发了一个模型来预测可测量的细胞,基于记者属性,成像参数和SNR.

主要成果:

  • 对于相似的光子速率,2P激发要求每单元显著更高的照明功率,而不是1P.
  • 使用当前的技术,在小鼠皮质中的2P电压成像 (1kHz带宽,200mW激光,500μm深度,SNR 10) 可以成像最多100个神经元.

结论:

  • 现有的GEVI表现出适度的电压敏感性,在2P电压成像中创建了射击噪声和光损伤之间的权衡.
关键词:
射击噪声 射击噪声 射击噪声两个光子的两个光子.电压成像 电压成像

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  • 实现深度数百个神经元的高SNR2P成像,需要在GEVIs或创新的成像方法中进行实质性改进.