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Updated: Jun 17, 2025

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
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RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

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内生全长人体特异性LINE-1RNA的北方斑点

Maisa I Alkailani1

  • 1College of Health and Life Sciences, Hamad Bin Khalifa University, Qatar Foundation, Doha, P.O. Box 34110, Qatar.

Biology methods & protocols
|August 14, 2024
PubMed
概括

检测活跃的LINE-1RNA是很困难的. 这项研究介绍了一种用于北方涂抹的新RNA探针方法,改善了基因组中活性LINE-1元素的检测.

关键词:
在 L1-Hs 的情况下.在RNA探测器中使用RNA探针.在北方的 blot blot.无线电标记是指无线电标记.在反向转换中进行反向转换.

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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
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