通过FEM1B的停止密码子阅读来调节细胞循环的类人特异性调节
Md Noor Akhtar1, Anumeha Singh1, Lekha E Manjunath1
1Department of Biochemistry, Indian Institute of Science, Bengaluru 560012, India.
Journal of cell science
|August 14, 2024
概括
停止密码子读透 (SCR) 调节FEM1B的表达,产生一种不稳定的蛋白质. 这种在人类和猿类中新发现的机制通过控制FEM1B水平,影响细胞循环调节.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 细胞生物学 细胞生物学
背景情况:
- FEM1B 作为 CRL2 E3 泛基因结合酶复合物的关键组成部分,调解蛋白质降解.
- 调节蛋白质水平对于细胞过程至关重要,包括细胞循环.
研究的目的:
- 通过停止编码子读透 (SCR) 调查FEM1B表达的调节.
- 阐明FEM1B SCR的机制和进化起源及其对细胞过程的影响.
主要方法:
- 利用CRISPR基因编辑创建缺乏FEM1B SCR cis调节区域的细胞.
- 分析了改变FEM1B表达对下游标,如SLBP和组织蛋白的影响.
- 进行了进化分析,以确定FEM1B SCR的物种特异性.
主要成果:
- 发现停止密码子读透 (SCR) 产生了一个C端扩展的不稳定的FEM1B异形.
- 在FEM1B 3'UTR中确定了一个81核酸区,该区域对于SCR和扩展产品的降解至关重要.
- 为了取消SCR的CRISPR编辑导致FEM1B增加,SLBP和胰岛素表达减少,细胞周期延迟.
- 发现FEM1B SCR特定于Hominini部落 (包括人类和黑猩猩).
结论:
- FEM1B的表达是由最近进化的停止密码子读透 (SCR) 机制调节的.
- 这种SCR过程导致扩展FEM1B异形的降解,从而减轻细胞周期的负调节.
- 这些发现突出了影响灵长类动物细胞周期进展的新型基因表达控制层.
更多相关视频
08:33Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
Published on: December 5, 2017
14.2K
10:54Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
Published on: September 17, 2012
10.5K
相关概念视频
01:23Negative Regulator Molecules
35.2K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.2K
01:28The Cell Cycle Control System
2.7K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
2.7K
02:39Positive Regulator Molecules
5.4K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.4K
01:27Molecular Factors Affecting Cell Division
3.0K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.0K
02:34Inhibition of Cdk Activity
4.7K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.7K
02:37DNA Damage can Stall the Cell Cycle
9.1K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.1K