相关实验视频

Updated: Jun 17, 2025

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

差异性miR-195-5p及其在动脉狭窄发展中的潜在作用

Haining Gao¹, Liansheng Zhao¹, Xuemei Cao¹

¹Department of Neurology, Nanjing Qixia District Hospital, Nanjing, China.

|August 14, 2024

概括

血清miR-195-5p显示为动脉狭窄症 (CAS) 的生物标志物,有助于诊断和预测中风风险. 这种微RNA还影响血管光滑肌肉细胞的行为,影响疾病的进展.

科学领域:

生物化学生化学
分子生物学分子生物学
心血管研究研究心血管研究

背景情况:

动脉狭窄 (CAS) 是大脑缺血事件 (CIE) 的主要原因之一.
早期检测和风险分层对于管理CAS患者和改善结果至关重要.
确定CAS的新生物标志物对于更好的患者管理至关重要.

研究的目的:

为了确定CAS的新生物标志物.
研究miR-195-5p在血管光滑肌细胞 (VSMC) 增殖和迁移中的作用.

主要方法:

在112名CAS患者和65名使用RT-qPCR的对照组中量化了血清miR-195-5p水平.
通过ROC曲线分析评估诊断准确性 (AUC=0.897).
使用卡普兰-梅尔和考克斯回归来评估预后意义;在体外研究中使用CCK-8和Transwell试验.

主要成果:

在CAS患者中,血清miR-195-5p水平显著升高,与狭窄症的严重程度相关.
miR-195-5p具有很高的诊断潜力,敏感度为71.4%,特异性为95.4%.
较高的miR-195-5p水平预测了较高的CIE风险,并促进了VSMC的扩散和迁移.

结论:

miR-195-5p是CAS的一个有前途的诊断和预后生物标志物.
miR-195-5p通过增强VSMC的扩散和迁移,可能有助于CAS的进展.

关键词:

冠状动脉狭窄症是什么临床意义临床意义发展发展发展发展发展.miR-195-5p-5p的使用情况.

更多相关视频

Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice

Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice

Published on: January 13, 2012

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

Published on: June 22, 2010

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...