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Updated: Jun 17, 2025

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Published on: March 19, 2016

在毛细血管微样本中进行血栓细胞计数的自动化方法.

Caroline Vasard Boesen¹, Vibeke Staun Christensen¹, Klaus Rosenkilde Jensen¹

¹Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark.

International journal of laboratory hematology

|August 14, 2024

概括

这项研究提出了一种自动化,低体积的方法来计算毛细血管血液中的血栓细胞数量. 这种新方法提高了分析质量,缩短了周转时间,为成人和儿科样本提供了可行的替代方案.

关键词:

在Sysmex中,我们使用的是Sysmex.生物化学生物化学血液组成部分血液成分新生儿血液学新生儿血液学血小板血小板是血小板细胞减少症 (Thrombocytopenia) 是一种

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科学领域:

血液学血液学血液学
临床化学临床化学
实验室医学实验室医学

背景情况:

为高效的实验室诊断,开发用于毛细血管血液中的血栓细胞计数的自动化,低体积的方法至关重要.
Sysmex预稀释 (PD) 模式为此类进步提供了一个潜在的平台.

研究的目的:

开发和评估一种自动化,低体积的血小板计数方法,使用毛细血管血液与Sysmex PD模式进行计数.
评估这种新方法的分析质量,预分析因素和周转时间.

主要方法:

微样 (50微升血液) 被准备好并重新悬浮在300微升DCL CellPack中.
使用阻抗 (PLT-I) 和光 (PLT-F) 通道进行了血小板计数.
不准确性,偏差和预分析因素 (皮肤穿孔,储存,气动管运输) 在394多个成年微样本中进行了评估,并与儿科样本进行了比较.

主要成果:

使用PLT-F的自动化方法显示不精确度为1.1%-3.7%,偏差为10.1%.
在皮肤穿孔后,偏差为8.1%,不准确为1.9%. 在室温下4小时后和气动管运输后,计数保持稳定.
在PD模式中,在PLT-F通道中的儿科微样本的偏差为13.0%. 与手动方法相比,自动化方法的不精确度明显降低,周转时间缩短.

结论:

自动化微样本方法为血小板细胞测量提供了低容量,高效的替代方案.
这种方法证明了在儿科样本分析中的实用性和应用潜力.