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Updated: May 10, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
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从真菌中采集八环环类基因组的碎片导向采集

Yalong Zhang1, Lin Wu1, Tyler A Kerr2

  • 1Departments of Chemical and Biomolecular Engineering, University of California, Los Angeles, California 90095, United States.

Journal of the American Chemical Society
|August 14, 2024
PubMed
概括

基因组挖掘使用功能化片段发现了一种新的生物合成策略. 这导致了八环素A的发现, 这是一种具有前所未有的八环结构的复杂天然产品.

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DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...