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相关概念视频

Master Transcription Regulators02:23

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
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Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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RAPGEF1 (C3G) 转录的发育和组织特异表达,具有编码具有预测调节功能的无序段的外显子.

Archana Verma1,2, Abhishek Goel1, Niladri Koner1

  • 1CSIR-Centre for Cellular & Molecular Biology, Uppal Road, Habsiguda, Hyderabad, 500 007, India.

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概括

研究人员发现了RAPGEF1 (C3G) 蛋白质的新型,通过替代拼接生成. 这些新的RAPGEF1异型在组织中表现出多种表达,并在发育过程中影响蛋白质活性.

关键词:
阿尔法折叠是什么意思替代拼接是一种替代拼接.在C3G中,它是C3G.停靠对接 停靠对接本质上是无序的区域.较低的德国.在RAPGEF1中使用.富含血清素的丰富的血清素.

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科学领域:

  • 分子生物学分子生物学
  • 基因规则 基因规则
  • 蛋白质分离形态 蛋白质分离形态

背景情况:

  • RAPGEF1 (C3G) 是一种关氨酸核酸交换因子,对小鼠胚胎发育至关重要.
  • 它调节基因表达和细胞骨组织,影响细胞增殖和分化.
  • 之前的研究预测了多个RAPGEF1转录,但它们的组织特异性表达仍然未被调查.

研究的目的:

  • 在各种小鼠组织中研究RAPGEF1异型的详细表达模式.
  • 识别新的RAPGEF1转录,并了解它们在蛋白质调节中的作用.

主要方法:

  • 对RAPGEF1拼接热点的分析,特别是涉及外子-3和外子12-14的热点.
  • 在特定器官 (心脏,大脑,丸,骨肌肉) 中使用磁带外显子检测未注释的转录.
  • 在结构分析 (AlphaFold) 和对接研究 (LZerD) 预测异型体的功能影响.

主要成果:

  • 在两个主要热点发生拼接后,确定了全长的RAPGEF1异型.
  • 在心脏,大脑,丸和骨肌肉中发现了不可预测的转录,其中包括磁带外型.
  • 在肌细胞和胚胎干细胞的分化过程中观察到异型切换;盒子外子编码一种可酸化,无序的,影响蛋白相互作用和催化活性.

结论:

  • 证明了新型RAPGEF1异型在不同组织和发育阶段的表达.
  • 卡塞特外子体的纳入代表了调节RAPGEF1活动的新机制.
  • 这些发现提供了关于RAPGEF1功能在各种生物环境中的复杂调节的见解.