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Updated: May 5, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
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Published on: October 1, 2014

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多重粒子追踪:一种探测细菌细胞外囊泡生物相关运动的方法

Darby Steinman1, Robert D Kirian1, Hannah C Zierden2,3,4

  • 1Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA.

Methods in molecular biology (Clifton, N.J.)
|August 14, 2024
PubMed
概括

细菌细胞外囊泡 (bEVs) 是用于细胞通信和药物输送的多功能纳米粒子. 本研究详细介绍了追踪生物屏障中的bEV运动的方法ex vivo.

关键词:
细菌细胞外囊泡 (bEVs) 是一种细菌细胞外囊泡.细菌膜囊泡 (bMVs) 是一种细菌膜囊泡.膜囊泡 (MVs) 是一种多个粒子跟踪追踪.外膜囊泡 (OMVs) 是一种

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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