Zfp697是一种RNA结合蛋白,可以调节骨肌肉的炎症和重塑
Jorge C Correia1, Paulo R Jannig1, Maya L Gosztyla2,3,4
1Molecular and Cellular Exercise Physiology, Department of Physiology and Pharmacology, Biomedicum, Karolinska Institutet, Stockholm SE-171 77, Sweden.
概括
新发现的Zfp697蛋白调节了受伤后骨肌肉的修复. 其有针对性的去除会损害肌肉再生和功能恢复,突出显示其在组织重塑中的关键作用.
科学领域:
- 肌肉生物学 肌肉生物学
- 再生医学是一种再生医学.
- 分子生物学分子生物学
背景情况:
- 骨肌肉缩是患病和死亡的重要风险因素,与不使用,慢性疾病和衰老有关.
- 肌肉缩或受伤恢复期间的组织重塑涉及肌肉纤维,卫星细胞和免疫细胞之间的复杂相互作用.
研究的目的:
- 调查未表征的基因和蛋白质Zfp697在骨肌肉重塑和再生中的作用.
- 为了确定Zfp697是否是一种损伤诱导的肌肉恢复调节器.
主要方法:
- 在肌肉缩或受伤后恢复期间,对小鼠和人类中Zfp697/ZNF697表达的分析.
- 研究持续Zfp697表达对小鼠肌肉中的基因表达和免疫细胞活性的影响.
- 利用小鼠的肌纤维特异性Zfp697遗传除来评估其对肌肉损伤反应和功能恢复的影响.
- 检查Zfp697在干扰素马反应中的作用及其作为RNA相互作用蛋白的功能.
主要成果:
- 在小鼠和人类的肌肉恢复过程中,Zfp697/ZNF697的表达暂时上调.
- 持续的Zfp697表达会诱导化学分泌,免疫细胞的招募,以及小鼠肌肉中的细胞外矩阵重塑.
- 对Zfp697的肌纤维特异性切除损害了对肌肉损伤的炎症和再生反应,损害了功能恢复.
- Zfp697被确定为肌肉细胞中干扰素马反应的关键调解剂,并作为具有众多miRNA标的RNA相互作用蛋白质起作用.
结论:
- Zfp697是一种损伤诱导的骨肌肉重塑和再生的调节剂.
- Zfp697集成细胞-细胞通信通路,对于有效的组织修复至关重要.
- 这些发现确定Zfp697是恢复受伤后肌肉功能的关键因素.
相关概念视频
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Formation of Muscle Fibers from Myoblasts
4.8K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
4.8K
NF-κB-dependent Signaling Pathway
7.4K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.4K
RNA Splicing
56.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.2K
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Regulation of Expression at Multiple Steps
876
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
876


