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相关实验视频

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Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
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使用滚动微针电极阵列进行体内核酸输送的协议.

Pengfei Wu1, Tongren Yang2, Dong Huang3

  • 1School of Life Science, Advanced Research Institute of Multidisciplinary Science, Key Laboratory of Molecular Medicine and Biotherapy, Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Beijing Institute of Technology, Beijing 100081, China.

STAR protocols
|August 14, 2024
PubMed
概括

我们开发了滚动微针电极阵列 (RoMEA) 以实现高效,低损伤的皮肤电穿孔. 这种设备可以在身体大面积上进行增强的核酸输送.

关键词:
生物物理学的生物物理学.生物技术和生物工程 生物技术和生物工程分子生物学分子生物学

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科学领域:

  • 生物医学工程 生物医学工程
  • 分子生物学分子生物学
  • 皮肤病学 皮肤病学

背景情况:

  • 电穿孔是一种提高细胞膜透性以吸收分子的方法.
  • 目前的电穿孔方法可能是低效的,或者会造成严重的皮肤损伤.

研究的目的:

  • 开发和评估一种新型设备,即滚动微针电极阵列 (RoMEA),用于高效和低损坏的电穿孔.
  • 为了证明RoMEA在体内核酸转移扩大皮肤区域的应用.

主要方法:

  • 设计,制造和组装RoMEA设备,采用密集排列的微针电极阵列和滚动结构.
  • 应用RoMEA用于体内电解,以创建均的皮肤微孔.
  • 使用RoMEA设备将核酸传染到皮肤中.

主要成果:

  • RoMEA 装置成功地创建了统一的皮肤微孔.
  • 核酸的高效和低损害的转移在身体的延伸区域实现.
  • 为设备使用和执行提供了详细的协议.

结论:

  • 滚动微针电极阵列 (RoMEA) 是一种有效的工具,可以增强通过皮肤传递核酸的效果.
  • 罗米亚 (RoMEA) 提供了一种有前途的方法,用于高效,大面积,低损坏的电 in vivo.
  • 这项技术在基因疗法和其他通过皮肤递送系统中具有潜在的应用.