单细胞分析元素含量通过激光剥离-感应合等离子体质谱学
Yu-Ki Tanaka1, Yasumitsu Ogra1
1Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo, Chiba 260-8675, Japan.
Journal of the American Society for Mass Spectrometry
|August 14, 2024
概括
使用激光剥离感应合等离子体质谱法 (LA-ICP-MS) 进行单细胞元素分析,揭示了神经元类细胞分化过程中必需矿物质的增加. 更大,更差异化的细胞显示出更高的元素含量,表明它们在以后的分化阶段的重要性.
科学领域:
- 分析化学 分析化学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 单细胞层面的元素分析是一种新兴的技术.
- 诱导合等离子体质谱 (ICP-MS) 与激光切除 (LA) 结合,可以根据大小和形态学对单细胞进行歧视性分析.
研究的目的:
- 为了评估单细胞内元素含量在老鼠上腺染细胞细胞分化成神经元类细胞过程中的变化.
- 为了将元素含量与细胞分化的程度相关联.
主要方法:
- 激光切除-诱导合等离子体质谱法 (LA-ICP-MS) 用于单细胞的元素分析.
- 用免疫光染色对微管相关蛋白2进行成像细胞计量 (Map2),以评估差异化.
- 细胞大小,光强度和元素含量之间的相关性分析.
主要成果:
- 七种必需矿物质在分化后增加了2-3倍.
- Map2表达在细胞之间有所不同,较不成熟的细胞呈现较低的光强度.
- 与较小,较不成熟的细胞相比,更大,更成熟的细胞表现出明显更高的元素含量.
结论:
- 在神经元分化过程中,元素含量显著增加.
- 基本矿物质对于细胞分化的后期阶段至关重要.
- LA-ICP-MS是分析分化细胞元素动态的一个有价值的工具.
更多相关视频
相关概念视频
Atomic Emission Spectroscopy: Lab
152
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
152
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
676
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
676
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle
556
Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
556
Atomic Emission Spectroscopy: Overview
1.7K
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
1.7K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation
201
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
201
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
421
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
421


