相关实验视频

Updated: Jun 17, 2025

Microwave-driven Synthesis of Iron Oxide Nanoparticles for Fast Detection of Atherosclerosis

Microwave-driven Synthesis of Iron Oxide Nanoparticles for Fast Detection of Atherosclerosis

Published on: March 22, 2016

反应性氧物种-响应性纳米粒子输送小干扰性核糖酸向动脉样硬化嗅觉受体2的治疗治疗神经硬化

Huaner Ni^1,2, Hui Zhou¹, Xin Liang¹

¹State Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, Nanjing 210023, China.

|August 14, 2024

概括

这项研究介绍了一种用于动脉样硬化 (AS) 的新型神经解剖纳米平台. 它使用小干扰性核糖核酸 (siRNA) 向巨细胞中的嗅觉受体2 (Olfr2),有效治疗AS,并通过光声成像进行诊断.

科学领域:

心血管研究研究心血管研究
纳米医学是一种纳米医学.
分子生物学分子生物学

背景情况:

动脉样硬化 (AS) 是一种由动脉脂质斑块标志着的慢性炎症性疾病.
小干扰核糖核酸 (siRNA) 疗法为AS提供精确的基因抑制,但面临着有限的巨细胞标.
嗅觉受体2 (Olfr2) 是AS斑块形成的新发现的潜在目标.

研究的目的:

开发和评估一个用于针对性地将抗Olfr2 siRNA (si-Olfr2) 输送到动脉样硬化病变中的巨细胞的光纳米平台.
为了降低Olfr2表达,用于AS治疗,并使用光声成像 (PAI) 进行诊断.

主要方法:

针对巨细胞的抗Olfr2 siRNA (si-Olfr2) 的设计.
开发一种响应活性氧物种 (ROS) 的纳米载体系统,用于封装si-Olfr2.
在第二个近红外 (NIR-II) 窗口中使用光声成像 (PAI) 进行诊断.

主要成果:

在动脉样硬化斑块内的巨细胞中有效下调Olfr2表达.
抑制含有3 (NLRP3) 炎症酶激活和互白素-1β (IL-1β) 分泌的NLR家族皮林域.
减少动脉样硬化斑块的形成,脂质沉积,巨细胞的亡和死角区域,以及减轻炎症.

结论:

这项研究展示了一种用于针对性地将si-Olfr2输送到损伤性巨细胞的theranostic纳米平台的概念证明.
这个平台显示出对动脉样硬化的诊断和治疗都有潜力.
这些发现强调了Olfr2作为AS的可行的治疗点.

关键词:

在NIR-II PAI中,在奥尔弗雷德2 动脉样硬化的治疗治疗 siRNA的传递方式他们是天文学家.

更多相关视频

Author Spotlight: Innovative Cancer Therapies with Iron Oxide Nanoparticles for Glioblastoma Treatment

Author Spotlight: Innovative Cancer Therapies with Iron Oxide Nanoparticles for Glioblastoma Treatment

Published on: September 27, 2024

A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System

A Positioning Device for the Placement of Mice During Intranasal siRNA Delivery to the Central Nervous System

Published on: August 15, 2019

相关概念视频

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...