长时间读取的测序揭示了由与细菌宿主进行遗传交换驱动的广泛的肠道菌体结构变异
Senying Lai1,2,3, Huarui Wang4, Peer Bork5,6,7
1Department of Neurology, Zhongshan Hospital and Institute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai, China.
Science advances
|August 14, 2024
概括
研究人员在人类肠道菌群中发现了14438种遗传变异或结构变异 (SV). 这些SV与抗生素耐药性和菌体和细菌之间的水平基因转移 (HGT) 有关.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 病毒学 病毒学
背景情况:
- 遗传变异对于理解菌体进化和功能至关重要.
- 人类肠道体有着显著的遗传多样性.
- 结构变异 (SVs) 代表了菌体中基因变异的关键类型.
研究的目的:
- 为了研究人类肠道菌群中的微细基因变异,特别是SVs.
- 为了确定肠道菌体SVs的流行率和特征.
- 探索这些菌体SVs的功能影响和起源.
主要方法:
- 在91个个体的样本上进行了病毒丰富的长读元基因组测序.
- 创建了一个对非冗余菌体SVs的全面目录.
- 生物信息分析被用来识别基因丰富,与细菌片段的同质性,以及水平基因转移 (HGT) 机制.
主要成果:
- 在人类肠道菌群中,共发现了14438个非冗余的菌体SV.
- 这些SV主要富含与重组,DNA甲基化和抗生素耐药性相关的基因.
- 很大一部分菌体 SV 序列与细菌碎片呈现同质性,这表明广泛的水平基因转移 (HGT).
- 在特定的菌体-细菌体对之间观察到基因交换,温带菌体与细菌染色体的相互作用频率高于毒性菌体.
结论:
- 这项研究详细介绍了人类肠道菌群的遗传景观,强调了SVs.的作用.
- 体SV涉及抗生素耐药性等重要功能,并积极参与与细菌的遗传交换.
- 了解这些遗传交换,特别是在温带菌体和它们的细菌宿主之间,是破译肠道微生物群中菌体-细菌相互作用的关键.
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