分析传统发酵酸肉的结构组成和抗氧化活性
Dezhen Meng1, Lijun Zhao1, Yaodi Zhu1
1College of Food Science and Technology, Henan Agricultural University, Zhengzhou, 450002, PR China; International Joint Laboratory of Meat Processing and Safety in Henan Province, Henan Agricultural University, Zhengzhou, 450002, PR China.
Food chemistry
|August 14, 2024
概括
较小的酸肉 (<1kDa) 显示出强烈的抗氧化作用. 它们的分子量,氨基酸概况和结构是这种增强的抗氧化活性的关键,在体外和体内.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生化学
- 营养科学 营养科学
背景情况:
- 中国传统的发酵酸肉具有独特的风味和营养益处.
- 的抗氧化特性受其分子量,氨基酸成分和结构特征的影响.
研究的目的:
- 研究酸肉的分子量,氨基酸组成,结构和抗氧化活性之间的关系.
- 为了确定负责显著抗氧化作用的的特定特征.
主要方法:
- 基于分子量 (<1 kDa) 的分离.
- 氨基酸组成和抗氧化剂活性分析 (体外和体内测试).
- 光谱技术 (UV-Vis,拉曼) 用于分析结构特征和微环境极性.
- 对二次结构的评估 (β转,α螺旋内容).
主要成果:
- 分子重量<1kDa的体在体外和体内表现出显著的抗氧化活性.
- 较小的分子量与较高的抗氧化氨基酸含量相关 (p <0.05).
- 结构分析显示,紫外线吸收峰值降低,吸收在284.5纳米处蓝移,微环境极性增加,提升了氨酸和酸氨基酸的拉曼峰值,以及富含β转和低α螺旋的二级结构.
结论:
- 分子重量是决定酸肉的抗氧化能力的关键因素.
- 较小的增强抗氧化活性归因于它们的氨基酸形状和特定的结构构造,包括增加的β-turn含量.
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