Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Visible light reprograms MSCs and T cells into tumor-suppressive states via OPN4-mediated epigenetic remodeling.

Acta pharmacologica Sinica·2026
Same author

Loss of miR-204 Drives NPTX1-Dependent Mitochondrial Dysfunction and Neuronal Degeneration in Alzheimer's Disease.

Cellular and molecular neurobiology·2026
Same author

Sequential vascular and neural changes underlying the progression of patellar tendinopathy.

Journal of orthopaedic surgery and research·2026
Same author

A novel glutathione S-transferase Gtt2 class from the marine toxic dinoflagellate Alexandrium pacificum and its roles in algicide exposures.

Ecotoxicology and environmental safety·2026
Same author

Development of a Sandwich-Type <i>sxtA4</i> Electrochemical Biosensor for Proactive Environmental Monitoring of STX-Producing Microalgae.

Biosensors·2026
Same author

Nature of Emergent Moiré Excitations in MoSe<sub>2</sub>/WS<sub>2</sub> Moiré Superlattices.

Nano letters·2026

相关实验视频

Updated: Jun 17, 2025

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.3K

高度增强的Hg2+检测使用优化DNA和基于双咖啡环效应的SERS地图.

Joohyung Park1, Kyunghwan Chai1, Woong Kim2

  • 1Department of Biomechatronics Engineering, Sungkyunkwan University, Suwon, 16419, Republic of Korea.

Biosensors & bioelectronics
|August 14, 2024
PubMed
概括

一个新的表面增强的拉曼散射 (SERS) 传感器有效地检测离子 (Hg2+) 使用优化的DNA探针和咖啡环效应. 这种高度敏感的方法提供了可靠的环境和食品安全监测.

关键词:
咖啡环效应是咖啡环效应.优化DNA优化 DNA优化这种离子是Hg离子.一个真实的样本样本.在SERS地图上,SERS地图.

更多相关视频

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.3K
Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril
10:02

Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril

Published on: October 3, 2020

3.8K

相关实验视频

Last Updated: Jun 17, 2025

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

20.3K
Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

3.3K
Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril
10:02

Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril

Published on: October 3, 2020

3.8K

科学领域:

  • 环境科学 环境科学
  • 分析化学 分析化学
  • 生物技术是生物技术.

背景情况:

  • 离子 (Hg2+) 是高度有毒的重金属,对人类健康和生态系统构成重大风险.
  • 通过食物链的生物积累需要在现实的条件下使用敏感的检测方法.

研究的目的:

  • 开发一种高度敏感和选择性的表面增强拉曼散射 (SERS) 传感器,用于检测离子 (Hg2+).
  • 创建一个用户友好的方法来评估环境和食品样本中的污染.

主要方法:

  • 设计了一种选择性DNA探针,通过胆氨酸-胆氨酸不匹配来准Hg2+ .
  • 利用双咖啡环效应来集中DNA探头,增强SERS信号.
  • 采用SERS光谱仪进行定量分析,并创建了披萨形状的SERS映射图像.

主要成果:

  • 获得了特殊的灵敏度,Hg2+的低检测极限为208.71 fM.
  • 对Hg2+比其他金属离子具有较高的选择性.
  • 在现实世界样本中成功检测到Hg2+,包括饮用水,自来水,头金枪鱼和金枪鱼沙米.

结论:

  • 开发的SERS传感器为敏感的Hg2+检测提供了一个有希望,可靠和用户友好的方法.
  • 这种方法在环境监测和通过检测污染来确保食品安全方面具有重大潜力.