急性寒冷暴露的时间决定了UCP1和FGF21的表达 - - 热环境,热调节反应和外围时钟之间的可能相互作用
Phong Kt Chau1, Elin Ryan1, Knut Tomas Dalen2
1Division of Work Psychology and Physiology, National Institute of Occupational Health (STAMI), Oslo, Norway.
Journal of thermal biology
|August 14, 2024
概括
身体在暴露在寒冷中调节温度的能力取决于一天中的时间. 这种昼夜节律会影响身体对寒冷的反应,影响热感知,并可能改变工作安全.
科学领域:
- 时间生物学 时间生物学
- 生理学 生理学 生理学
- 代谢过程中的代谢.
背景情况:
- 温度调节对于维持热平衡至关重要,并且已知与昼夜周期同步.
- 日间时间对急性寒冷暴露的生理反应的影响仍然不完全理解.
研究的目的:
- 调查一天中的时间是否影响小鼠对环境寒冷暴露的急性反应.
- 探索昼夜节律在调节温度调节反应和寒冷压力期间的热感知中的作用.
主要方法:
- 在适应热中性 (27°C) 后,小鼠在不同的昼夜阶段 (LIGHT,DARK,D+L) 暴露在寒冷 (15°C) 中8小时.
- 测量包括棕色脂肪组织 (BAT) 中的UCP1mRNA和蛋白质,血FGF21水平,BAT昼夜钟基因表达和行为热敏度 (热梯度测试).
- 进行了背部根结节 (DRG) 体感神经元的RNA测序,以分析转录变化.
主要成果:
- 在BAT中冷诱导的UCP1和FGF21表达在LIGHT阶段明显高于DARK阶段,在D+L阶段的中间水平.
- 在BAT中,急性寒冷暴露改变了核心昼夜钟基因的mRNA表达模式,但在肝脏中没有.
- 行为测试显示,在D+L阶段暴露于寒冷后,寒冷敏感性降低,这表明热感知发生了变化.
结论:
- 暴露于急性寒冷会对温度调节通路产生特定时间的影响,包括代谢和荷尔蒙反应.
- 在BAT和感官神经元中,生理时钟的组成部分在调节这些对寒冷的时间依赖反应方面发挥着作用.
- 研究结果表明,热感知受到昼夜生理学的影响,这对寒冷环境中的轮班工作有影响.
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