在智利中部的海鸟中产生ESBL的大肠杆菌的流行和共享
Tania Suarez-Yana1, Marilia Salgado-Caxito2, Juliette Hayer2
1Doctorado en Medicina de la Conservación y Centro de Investigación para la Sustentabilidad, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago 8320000, Chile.
The Science of the total environment
|August 14, 2024
概括
智利的海鸟携带并传播抗微生物耐药性大肠杆菌,与迁徙的富兰克林海相比,居住的海和秘鲁比较普遍. 这突显了耐药性基因在鸟类物种中的环境传播.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 鸟类生态学 鸟类生态学
背景情况:
- 抗菌素耐药性 (AMR) 是一个重要的全球健康问题,在自然生态系统中越来越多的报道.
- 海鸟是被公认的扩展光谱β-乳糖酶产生大肠杆菌 (ESBL-E. coli) 的储库.
- 海鸟种类的食和繁殖行为的变化可能会影响ESBL-E. coli的循环.
研究的目的:
- 为了比较ESBL-E. coli在居民海 (Larus dominicanus),迁徙富兰克林海 (Larus pipixcan) 和特有秘鲁 (Pelecanus thagus) 的流行率和遗传特征.
- 调查智利中部的洪堡流生态系统 (HCE) 中ESBL-E. coli的潜在物种间传播.
- 在这些海鸟种群中识别特定的AMR基因,毒性因子和与ESBL-E. coli相关的移动遗传元素.
主要方法:
- 在2020年至2022年期间,从三种海鸟中收集和分析了699个新鲜便样本.
- 隔离和鉴定大肠杆菌ESBL-E. coli,随后对85个选定的分离物进行全基因组测序 (WGS).
- 在剩余的隔离物中通过PCR检测ESBL基因,以及对序列类型 (ST) 的WGS,AMR基因,病毒性基因和移动遗传元素 (MGE) 的检测.
主要成果:
- 与弗兰克林海 (15%) 相比,海 (46%) 和秘鲁 (34%) 的ESBL-E. coli患病率更高.
- 全基因组测序确定了41种ESBL-E.大肠杆菌ST,包括流行性克隆ST10,ST58,ST131和ST410.
- 在至少两种海鸟物种之间共享了八种ESBL-E. coli STs,植物遗传学分析表明克隆交换,特别是在食人类排泄物的物种中.
结论:
- 在HCE的海鸟,包括消耗人类排泄物的秘鲁,有助于ESBL-E. coli的传播.
- 观察到不同海鸟物种之间存在ESBL-E. coli的物种间传播和克隆交换的证据.
- 这项研究强调了海鸟在自然环境中作为AMR载体的作用,强调了需要"一个健康"的方法.
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