MYC转录因子PbrMYC8负面调节PbrMSL5的表达,以促进Pyrus中的花粉发芽
Mingliang Zhang1, Chao Tang1, Yu Li1
1Sanya Institute of Nanjing Agricultural University, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Key Laboratory for Horticultural Crop Breeding, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
International journal of biological macromolecules
|August 14, 2024
概括
小导电性的机械敏感通道 (MSL) 调节花粉发芽和细胞完整性. PbrMYC8抑制PbrMSL5,通过调节透压力和含量来促进梨花花粉的发芽.
科学领域:
- 植物生殖生物学 植物生殖生物学
- 分子植物生理学分子植物生理学
背景情况:
- 成功的花粉发芽对于开花植物的双重受精至关重要.
- 小导电性的机械敏感通道 (MSL) 在抑制花粉发芽和保持细胞完整性方面发挥作用.
- 在植物中调节MSL表达的分子机制尚不清楚.
研究的目的:
- 在梨 (Pyrus bretschneideri) 中识别和描述MSL基因.
- 阐明PbrMSL5在花粉发育和发芽中的作用.
- 揭示控制PbrMSL5表达的调节机制及其对花粉发芽的影响.
主要方法:
- 在珍珠中识别和表达MSL基因的分析.
- PbrMSL5.5的亚细胞局部化
- 使用Arabidopsis突变物进行补充测定.
- 反感性寡核酸治疗.
- 酵母的一混合,电泳运动转移试验 (EMSA) 和双路西法酶记者试验.
主要成果:
- 在梨子中确定了15个MSL基因,其中PbrMSL5在花粉发育过程中特别表达.
- PbrMSL5局限于血和细胞质,保持花粉细胞的完整性,并抑制发芽.
- PbrMSL5通过降低透压力和含量来抑制梨花花粉的发芽.
- PbrMYC8直接与N盒元素相互作用,抑制PbrMSL5的表达,从而促进花粉发芽.
结论:
- PbrMSL5是梨花花粉发芽和细胞完整性的关键调节者.
- PbrMYC8作为PbrMSL5的转录抑制剂,促进花粉发芽.
- 这项研究揭示了控制MSL基因表达的新型分子机制及其在植物繁殖中的作用.
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