在骨和关节感染中检测罕见的微生物,通过元基因组下一代测序
Hongxin Hu1,2,3,4, Haiqi Ding2,3,4, Jianhua Lyu1,2,3,4
1Department of Orthopaedics, Affiliated Hospital of Putian University, Putian, China.
Bone & joint research
|August 14, 2024
概括
下一代元基因组测序 (mNGS) 显著改善了在骨和关节感染 (BJIs) 中检测罕见病原体. 这种先进的诊断方法也导致更好的临床结果,包括减少并发症和更短的住院时间.
科学领域:
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
- 临床诊断 临床诊断 临床诊断
背景情况:
- 骨关节感染 (BJIs) 带来了诊断挑战,通常涉及罕见的病原体.
- 传统的微生物培养方法在检测所有致病原体方面存在局限性.
研究的目的:
- 评估BJI中罕见病原体的下一代元基因组测序 (mNGS) 的诊断产量.
- 评估mNGS对临床诊断,治疗和患者结果的影响.
主要方法:
- 在2015年1月至2021年12月期间治疗的235名BJI患者的回顾性分析.
- 仅接受微生物培养 (无mNGS) 的组与使用mNGS与微生物培养一起 (mNGS组) 的组之间的比较.
主要成果:
- 与没有mNGS组相比,mNGS组的罕见病原体检测率更高 (21.6%对10.2%).
- 在mNGS组的患者经历了较少的抗生素相关并发症,更短的住院时间,并改善了感染控制率.
- 这些好处在培养负BJI病例中尤为明显.
结论:
- 下一代元基因组测序 (mNGS) 增强了BJI中罕见病原体的检测.
- 实施mNGS可以改善临床结果,包括减少并发症和住院时间,特别是在具有挑战性的负培养场景中.
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