天然的深溶性溶剂,用于从人体血中同时衍生和微提取异化
Daria Meshcheva1, Firuza Krekhova1, Andrey Shishov1
1Department of Analytical Chemistry, Institute of Chemistry, Saint Petersburg State University, SPbSU, 7/9 Universitetskaya nab., St. Petersburg, 199034, Russia.
Analytica chimica acta
|August 14, 2024
概括
一种新的深度浸泡溶剂方法有效地分离和量化血中的异化. 这种个性化医疗方法有助于通过分析药物代谢变异性来定制结核病治疗.
科学领域:
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 个性化医学根据个体的药理动力学特征量身定制治疗方法.
- 结核病药物伊索尼亚齐德 (Isoniazid) 呈现出明显的个体间代谢变异性.
- 这种变化凸显了个性化治疗方案的必要性.
研究的目的:
- 开发一种用于分离和确定异化在人体血中的新方法.
- 为了利用一种深度的浸泡溶剂进行液-液微提取.
- 为结核病治疗提供个性化医疗方法.
主要方法:
- 开发一种用于液-液微提取的深度环氧溶剂 (硫醇和4-甲基甲).
- 同时将异化衍生为疏水性希夫基和提取.
- 高性能液体色谱与UV-Vis检测用于量化.
主要成果:
- 提出了一种新的天然深度环氧溶剂用于提取.
- 该方法实现了分别为20和60μg/L的检测和量化极限.
- 提取恢复率为95%,相对标准偏差<10%.
结论:
- 首次在自然的深度欧性溶剂阶段中证明了异化的衍生物化.
- 在没有昂贵的试剂或弹的情况下,成功分离和预先缩异化物.
- 通过质谱测量证实了船基形成.
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