甲基转录组分析表明,在人体肠道微生物群上,异马尔托/马尔托多糖类对人体肠道微生物群的益生菌作用在体外
Klaudyna Borewicz1,2, Bastian Hornung3,4,5, Fangjie Gu6,7
1Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
Scientific reports
|August 14, 2024
概括
异/多糖 (IMMP) 是一种新型的可溶性食纤维. 这项研究揭示了具有特定链接的IMMPs如何影响肠道微生物基因表达,并促进像Lactobacillus和Bifidobacterium这样的有益细菌.
科学领域:
- 微生物学 微生物学
- 营养科学 营养科学
- 生物化学 生物化学
背景情况:
- 异/多多糖 (IMMP) 是可溶性食纤维,具有已证明的前生物潜力.
- 以前的研究表明,IMMPs增加了肠道细菌,特别是乳酸菌,以及短链脂肪酸 (SCFA) 产量.
- IMMPs发挥其益生菌作用的确切机制尚不清楚.
研究的目的:
- 调查IMMPs与不同α-(1→6) 糖化链接对肠道微生物功能的影响.
- 为了阐明IMMPs的作用方式,使用metatranscriptomics.
- 为了确定参与IMMP降解的特定肠道细菌及其功能反应.
主要方法:
- 在体外发酵IMMPs与人类便注射剂.
- 转录基因分析用于评估微生物基因表达特征.
- 细菌社区动态和相对丰度的分析.
主要成果:
- 微生物群落的动态和基因表达因IMMP类型和α-(1→6) 链接含量而异.
- 细菌类,乳酸菌和双菌被确定为主要的IMMP降解剂.
- 这些细菌的基因表达增加与更高的α-(1→6) 联系相关,以及增强的SCFA合成途径.
结论:
- IMMP发酵改变了肠道微生物群落的结构和功能,有特定的联系推动了这些变化.
- 这项研究提供了关于IMMPs对有益肠道细菌的向调节的见解.
- 这些发现支持用于特定健康结果的益生菌的合理设计.
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