微生物无细胞DNA测序作为新生儿败血症常规诊断的补充
Julian Balks1,2, Silke Grumaz3, Sonia Mazzitelli3
1Division of Neonatology and Pediatric Intensive Care, Children's Hospital, University of Bonn, Bonn, Germany.
Pediatric research
|August 14, 2024
概括
下一代测序 (NGS) 在新生儿败血症诊断中显示出检测微生物无细胞DNA (mcfDNA) 的前景. 这种先进的方法可以提高病原体的识别与传统的血培养相比,帮助有针对性的治疗.
科学领域:
- 新生儿医学 新生儿医学
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 血流感染在新生儿学中构成了重大挑战.
- 传统的血液培养是缓慢的,需要大量的血液.
- 下一代测序 (NGS) 通过识别微生物无细胞DNA (mcfDNA) 来从小血样中快速检测病原体.
研究的目的:
- 为了评估DISQVER®-NGS.的诊断性能.
- 为了将NGS与怀疑败血症的新生儿的标准血液培养进行比较.
主要方法:
- 从怀疑败血症的新生儿收集血液培养和NGS样本的前景.
- 将患者分为败血症 (血培阳性),临床败血症 (培养阴性),疑似败血症和验证队列.
- 通过NGS检测mcfDNA的分析和与血液培养结果的比较.
主要成果:
- 在82个样本中,NGS在24个样本中检测到细菌,病毒或真菌mcfDNA.
- 在46名患者中,15名患者的血液培养结果呈阳性.
- DISQVER® 正确识别了9/15名血培阳性患者的病原体,其中包括两名抗生素耐药患者.
- 在七个样本中,NGS确定了血液培养中未检测到的细菌的mcfDNA.
结论:
- 通过检测mcfDNA,NGS有可能提高新生儿败血症诊断的敏感性.
- 解释NGS结果需要与临床数据和常规微生物学测试进行整合.
- NGS可能会改善病原体检测,并指导新生儿败血症的向治疗,尽管需要进一步验证.
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