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Updated: Jun 17, 2025

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在亚洲开发和评估一种用于亚洲Sporothrix globosa的快速诊断方法,使用定量实时PCR

Hanqing Geng1,2, Lele Sun1,2, Chuan Wang1,2

  • 1Hospital for Skin Diseases, Shandong First Medical University, Jinan, Shandong, China.

BMC infectious diseases
|August 14, 2024
PubMed
概括

一种新的定量PCR测定准确地识别了Sporothrix环球糖,这是快速诊断运动三症的关键步骤. 这种方法为临床环境提供了一种可靠的工具,以打击这种真菌感染的日益流行.

关键词:
球体菌体 (Sporothrix globosa) 是一个有机体.诊断 诊断 诊断 诊断 诊断定量PCR是一种定量PCR.

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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