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通过CITE-seq分析识别衰老细胞亚种群

Kotb Abdelmohsen¹, Krystyna Mazan-Mamczarz¹, Rachel Munk¹

¹Laboratory of Genetics and Genomics, National Institutes of Health (NIH), Baltimore, Maryland, USA.

|August 15, 2024

概括

与衰老相关的细胞衰老显示出多种细胞类型的多样性. 研究人员使用细胞表面蛋白识别出不同的衰老细胞群体,为与年龄相关的疾病提供了新的治疗点.

科学领域:

细胞和分子生物学细胞和分子生物学
免疫学免疫学免疫学
老年学是指老年学的学科.

背景情况:

细胞衰老是衰老和与年龄相关的疾病的一个关键因素.
了解衰老细胞异质性对于开发有效疗法至关重要.
鉴定衰老细胞的特定标记物是一个重大挑战.

研究的目的:

为了研究衰老细胞种群的异质性.
识别区分不同类型衰老细胞的细胞表面蛋白质.
探索老化相关病理的潜在治疗点.

主要方法:

质谱法用于识别复制性疲劳和电离辐射 (IR) 诱导的衰老细胞上的表面蛋白质.
单细胞CITE-seq (通过测序对转录体和表皮体的细胞索引) 用于详细分析细胞表面蛋白质表达.
进行了生物信息分析,以基于蛋白质标志物对不同的衰老细胞种群进行集群和表征.

主要成果:

根据细胞表面蛋白质配置文件确定了两个不同的IR诱导衰老细胞种群.
集群3衰老细胞表现出高水平的CD109和CD112.
集群0衰老细胞显示CD112,CD26,CD73,HLA-ABC,CD54,CD49A和CD44的高表达,代表增殖和G1期衰老细胞.

结论:

细胞表面蛋白质是区分衰老细胞异质性和子类的有价值标记.
在混合种群中,CITE-seq有效地区分衰老细胞.
这项研究为开发针对老化相关疾病的向治疗提供了基础.

关键词:

在CITE-seqq.细胞循环中的细胞循环.蛋白质组是一种蛋白质组.衰老是一种老化.单细胞转录组是一个单细胞转录组.表面蛋白质表面蛋白质表面ome 表面ome 表面ome

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