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调节动脉内密硬度通过血液流动的干扰来调节

Briana C Bywaters¹, Andreea Trache^2,3, Gonzalo M Rivera¹

¹Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, United States.

Experimental biology and medicine (Maywood, N.J.)

|August 15, 2024

概括

慢性地扰乱血液流动会使动脉内脏变硬. 抑制原交叉连接可以防止这种有害的硬,为动脉样硬化提供潜在的治疗点.

科学领域:

心血管生物学心血管生物学
生物物理学的生物物理.
血管力学血管力学

背景情况:

动脉样硬化病原包括动脉内脏,由内皮和子内皮基质组成.
由于血液流动 (d-flow) 受到干扰和动脉硬化造成的机械应激会导致内皮功能障碍.
物理力量对内心机械环境的确切影响尚未完全理解.

研究的目的:

调查抑制原交联是否可以减轻持续d流对intima机械性能不利影响.
分析d-flow诱导的亲密的急性和慢性机械变化.
为了确定原交联在d流引起的动脉硬化中的作用.

主要方法:

在小鼠中,通过左动脉 (LCA) 的部分绑定诱导d流,以右动脉 (RCA) 作为控制.
服用β-aminopropionitrile (BAPN),一种 lysyl氧化酶 (LOX) 抑制剂,以阻止原的交叉连接.
使用原子力显微镜 (AFM) 来测量面对鼻准备中的内刚度.
在不同的水凝硬度上评估人类大动脉内皮细胞 (HAEC) 硬度.

主要成果:

在雄性小鼠中,急性d流略微降低了内皮硬度;子内皮矩阵硬度没有受到影响.
慢性d-flow显著增加了内皮和子内皮基质的硬度,在两种性别.
在很大程度上,BAPN的使用可以防止慢性d-flow诱导的硬化.
与硬相比,HAEC显示软凝的硬度降低.

结论:

慢性地扰乱血液流动会导致动脉内脏的显著硬化.
抑制原交叉连接有效地防止d流诱导的内硬化.
准原蛋白交叉连接是一种潜在的策略,可以抵消动脉样硬化的血管硬.

关键词:

在BAPN中,你会发现.动脉硬性动脉硬性原子力显微镜的原子力显微镜.原蛋白的交叉连接干扰了血液的流动.它们的内皮质 (endothelium).lysyl 氧化酶的作用是什么亚内末质矩阵是一个亚内末质矩阵.

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