通过动态核偏振增强磁共振来检测生物标志物
Shizhen Chen1,2,3, Lei Zhang1,2, Sha Li1,2
1State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China.
National science review
|August 15, 2024
概括
动态核极化 (DNP) 增强磁共振成像 (MRI) 灵敏度,用于实时代谢监测. 响应性DNP药物检测生物标志物,从而改善疾病的诊断和治疗.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 分子成像学分子成像学
- 核磁共振是一种核磁共振.
背景情况:
- 超极化技术,特别是动态核极化 (DNP),显著提高核磁共振 (NMR) 和磁共振成像 (MRI) 的灵敏度.
- 溶解DNP (dDNP) 能够实时,体内监测代谢过程和高极化剂的生物分布,特别是在动物模型中.
- 生物标志物对瘤生长和转移至关重要;检测它们的病理变化是诊断和治疗疾病的关键.
研究的目的:
- 审查用于生物医学成像的超极化DNP分子生物响应剂的开发和应用.
- 探索DNP增强磁共振信号如何响应各种生物标记物,如pH,金属离子,酶和氧化还原过程.
- 为这些响应性DNP代理提供设计原则,目标选择和行动机制的见解.
主要方法:
- 综述了使用多种核的超极化DNP分子生物响应剂 (例如13C,15N,31P,89Y) 的最新进展.
- 分析DNP增强信号与特定生物标志物 (pH,金属离子,酶,氧化还原状态) 的相关性.
- 讨论这些响应性代理的设计策略和成像机制.
主要成果:
- 开发各种高极化DNP剂,对关键的生物和病理生物标志物产生反应.
- 证明DNP能够实时可视化与生物标志物相关的代谢和生理变化.
- 在DNP增强的MRI中信号变化与特定生物标志物活动之间建立了联系.
结论:
- 响应性DNP剂为细胞代谢和疾病生物标志物的非侵入性体内成像提供了强大的工具.
- 了解设计原理和响应机制对于推进基于DNP的生物医学成像至关重要.
- 未来的DNP剂的开发对改善疾病诊断和治疗监测具有显著的前景.
相关概念视频
01:15Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)
274
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
274
01:25Applications Of NMR In Biology
3.7K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
3.7K
01:12Double Resonance Techniques: Overview
192
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
Spin decoupling is usually achieved by...
192
01:07Nuclear Overhauser Enhancement (NOE)
646
Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the Nuclear Overhauser Enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring...
646
01:05Atomic Nuclei: Magnetic Resonance
639
The number of nuclear spins aligned in the lower energy state is slightly greater than those in the higher energy state. In the presence of an external magnetic field, as the spins precess at the Larmor frequency, the excess population results in a net magnetization oriented along the z axis. When a pulse or a short burst of radio waves at the Larmor frequency is applied along the x axis, the coupling of frequencies causes resonance and flips the nuclear spins of the excess population from the...
639
01:07Nuclear Magnetic Resonance (NMR): Overview
2.3K
Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
2.3K