基于生物信息学分析,研究不同喘亚型中与年龄相关的基因的功能
Xinning Liu1, Bing Li2, Shuya Liu3
1Central Laboratory, Clinical Laboratory and Qingdao Key Laboratory of Immunodiagnosis, Qingdao Hiser Hospital Affiliated of Qingdao University (Qingdao Traditional Chinese Medicine Hospital), Qingdao, 266034, China.
Heliyon
|August 15, 2024
概括
这项研究确定了不同类型的喘中与衰老相关的基因,揭示了它们在炎症中的作用. 特定的基因显示出对乙酸性和中性酸性喘的诊断潜力,提供了新的治疗点.
科学领域:
- * 基因组学和生物信息学
- * 免疫学和呼吸系统医学
背景情况:
- *喘是一种复杂的炎症性呼吸道疾病,具有不同的表型 (eosinophilic,neutrophilic, paucigranulocytic).
- *表型变异影响病原,临床表现,治疗反应和预后.
- * 越来越多地认为衰老是导致慢性疾病发展的因素,包括喘.
研究的目的:
- * 调查与衰老相关的基因在不同喘炎症表型中的作用.
- *根据与衰老相关的基因表达来确定潜在的诊断生物标志物和治疗点.
主要方法:
- *基因组丰富分析 (GSEA) 用于途径识别.
- *用于免疫细胞组成分析的CIBERSORT算法.
- *差异基因表达分析 (limma R包) 和生物信息学与与衰老相关的基因数据库的整合.
- *基因本体学 (GO),KEGG通路丰富,蛋白质相互作用 (PPI) 网络和受体操作特征 (ROC) 分析.
主要成果:
- *与衰老相关的差异性表达基因 (DEGs) 在酸性喘 (EA) 和中性喘 (NA) 中被确定.
- * 丰富分析表明这些DEGs参与了细胞因子介导的信号通路.
- *ROC分析显示,EA患者的3种与衰老相关的DEG和NA患者的12种DEG的诊断准确度高 (AUC>0.7).
结论:
- *与衰老相关的基因在特定喘炎症表型的发病过程中起着重要作用.
- *已确定与衰老相关的DEGs为表型特定的诊断生物标志物和向治疗提供了潜力.
- * 干预措施可以从解决衰老循环,代谢疾病和喘恶化中获益.
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