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肉提取物,甲和酸抑制HDAC 1活动
Halil Koyu1, Huseyin Istanbullu2, Sinem Ezgi Turunc Ozoglu3
1Department of Pharmaceutical Botany, Faculty of Pharmacy, Izmir Katip Celebi University, 35620, Cigli-Izmir, Turkey. halil.koyu@ikcu.edu.tr.
Food & function
|August 15, 2024
概括
cinnamon 提取物显示强烈抑制 histone deacetylase 1 (HDAC 1),这表明对癌症和神经退行等与HDAC相关的疾病具有潜在的治疗益处. 进一步研究肉.
科学领域:
- 生物化学和分子生物学
- 药理学 药理学是指药理学的学科.
- 自然产品化学 自然产品化学
背景情况:
- 基因组脱乙酶 (HDAC) 酶对细胞功能至关重要,并与癌症,炎症和神经退行等疾病有关.
- 自然产品越来越多地被研究其作为HDAC抑制剂的潜力,提供治疗途径.
- 从植物提取物中提取的食补充剂可能对慢性HDAC相关疾病提供好处.
研究的目的:
- 评估肉 (Cinnamomum cassia) 提取物及其关键化合物的HDAC 1抑制活性.
- 为了量化肉提取物中和黄的总含量.
- 为了分析不同肉提取物中 cinnamaldehyde 和 cinnamic 酸的度.
主要方法:
- 使用不同极性的溶剂提取肉皮.
- 总和黄含量的光谱测量量量化.
- 在UPLC-DAD和ESI-MS/MS分析中量化甲和酸.
- 使用IC50值测定HDAC1抑制活性.
主要成果:
- 乙醇和二甲提取物显示出最高的甲和酸含量.
- 肉精油 (IC50: 51.11 μg/mL) 和70%乙醇提取物 (IC50: 107.90 μg/mL) 显示出最强的HDAC 1抑制作用.
- 单个化合物,肉甲 (IC50:7.58μg/mL) 和肉酸 (IC50:9.15μg/mL),也显示出显著的抑制活性.
- 70%的乙醇提取物的有效性,尽管 cinnamaldehyde / cinnamic 酸水平较低,表明其他化合物有助于抑制HDAC 1.
结论:
- 肉树皮提取物,特别是精油和70%的乙醇提取物,具有显著的HDAC 1抑制特性.
- 肉甲和肉酸是肉HDAC 1抑制作用的关键因素.
- 这些发现支持Cinnamomum cassia提取物作为天然HDAC 1抑制剂的来源的潜力,用于针对HDAC相关疾病的产品.
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