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Updated: May 17, 2026

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
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Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

Published on: January 12, 2014

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一个化化学基因pH传感器用于成像蛋白质外细胞化.

Justine Coïs1,2, Marie-Laure Niepon2, Manon Wittwer1

  • 1Laboratoire des biomolécules, LBM, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, France.

ACS sensors
|August 15, 2024
PubMed
概括

我们开发了一种新的化学遗传pH传感器,pHluo-Halo-1,用于精确跟踪细胞过程中pH值的变化,如细胞外. 这种无洗成像方法确保了高信号选择性,克服了传统光探针的局限性.

关键词:
在HaloTag中使用HaloTag.化学遗传传感器的传感器异位细胞形成的表细胞化.化探测器的化探测器检测pH值的探头

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Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator11:54

Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator

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相关概念视频

pH Regulation in Cells01:28

pH Regulation in Cells

pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...